|
KMID : 0861020140290030079
|
|
Korea Journal of Herbology
2014 Volume.29 No. 3 p.79 ~ p.85
|
|
|
The Study of Cytokine Inhibitory Effect using Careswell
|
|
Ha Hyo-Cheol
Oh Sa-Rang
Lee Ji-Hye
Jung Ji-Wook
|
|
|
Abstract
|
|
|
Objectives : In this study, we attempted to evaluate the effects of Careswell on human mast cell-mediated allergy inflammation in vitro and pruritogen-induced scratching behavior in vivo.
Method : The Careswell was extract by distilled water. The anti-itching effects of Careswell were investigated on the compound 48/80 (50 μg/kg) or histamine (100 μg/kg) induced scratching behavior male ICR mice for 30 min by an observer blind. Terfenadine (10 mg/kg) was used as a positive control drug. The cell toxicity of Careswell was determined by 3-(4,5-dimethylthiazole-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The regulatory effect of Careswell on interleukin (IL)-6 and tumor necrosis factor (TNF) -α levels was determined by enzyme-linked immunosorbent assay (ELISA) in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) stimulated human mast cells (HMC-1). Also, we evaluated the effect of Careswell on PMACI induced the activation of Nuclear factor-kappa B (NF-κB) into nucleus by Western blot analysis.
Result : The results revealed that the oral administration of Careswell (200 mg/kg, p.o.) attenuated the compound 48/80 or histamine-induced scratching behavior in mice. We showed that Careswell significantly reduced the PMACI-induced the production of IL-6 (0.5-1 mg/ml) and TNF-α (0.1-1 mg/ml). Additionally, Careswell significantly inhibited the activation of NF-κB in PMACI-stimulated HMC-1.
Conclusion : Collectively, the findings of this study provide us with a novel insight into the pharmacological actions of Careswell as a potential molecule for use in the treatment of allergic inflammation diseases.
|
|
|
KEYWORD
|
|
|
Careswell, Inflammation, Human mast cells, Cytokine, NF-κB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|